date: 2025-12-01T08:49:53Z
pdf:unmappedUnicodeCharsPerPage: 0
pdf:PDFVersion: 1.7
pdf:docinfo:title: Microbial ecology of subsurface granitic bedrock: a humid?arid site comparison in Chile
xmp:CreatorTool: LaTeX with hyperref package
Keywords: 
access_permission:modify_annotations: true
access_permission:can_print_degraded: true
subject:  DOI: 10.1093/ismeco/ycaf199, ISME Communications, 5(1), 1, 4 11 2025. Abstract: Subsurface microorganisms face extreme challenges such as anoxic, xeric, and oligotrophic conditions. In igneous systems, nutrient limitation is critical, as biomass input relies on surface-derived fluids via tectonic fractures. Despite growing interest in subsurface habitats, little is known about ecosystems beneath arid landscapes, where surface water input is limited by the low annual precipitation. This study compares granitic subsurface environments beneath arid and humid surface ecosystems, highlighting the link between surface climate and subsurface biodiversity. DNA was extracted from granitic subsurface rocks recovered from two endmember sites along a north?south climate gradient in Chile?s Coastal Cordillera. Microbial communities inhabiting down to 55 m deep subsurface rocks were characterized using 16S rRNA amplicon and shotgun metagenomic sequencing. We identified an abundant and potentially active subsurface community below both climates dominated by heterotrophic bacteria, including Pseudarthrobacter, Janthinobacterium, and Pseudomonas. However, rare taxa affiliated with common chemolithoautrophs, e.g. Thiobacillus, Sulfuriferula, and Sulfuricurvum, were only observed in the arid subsurface, indicating increased oligotrophic conditions and reliance on inorganic electron donors in the deeper subsurface of the desert. Functional analysis revealed sulphur, hydrogen, and carbon monoxide as potential inorganic electron donors. These findings expand the current understanding of microbial life in the subsurface of granite rocks showing the influence of surface climate on nutrient conditions in the deeper subsurface, providing new insights into the extent and functional capacity of terrestrial subsurface habitats and their role in global biogeochemical processes. 
PDFVersion: 1.5
language: en
dcterms:created: 2025-11-26T11:25:51Z
Last-Modified: 2025-12-01T08:49:53Z
dcterms:modified: 2025-12-01T08:49:53Z
dc:format: application/pdf; version=1.7
title: Microbial ecology of subsurface granitic bedrock: a humid?arid site comparison in Chile
Last-Save-Date: 2025-12-01T08:49:53Z
pdf:docinfo:creator_tool: LaTeX with hyperref package
access_permission:fill_in_form: true
pdf:docinfo:keywords: 
pdf:docinfo:modified: 2025-12-01T08:49:53Z
meta:save-date: 2025-12-01T08:49:53Z
pdf:encrypted: false
dc:title: Microbial ecology of subsurface granitic bedrock: a humid?arid site comparison in Chile
modified: 2025-12-01T08:49:53Z
cp:subject:  DOI: 10.1093/ismeco/ycaf199, ISME Communications, 5(1), 1, 4 11 2025. Abstract: Subsurface microorganisms face extreme challenges such as anoxic, xeric, and oligotrophic conditions. In igneous systems, nutrient limitation is critical, as biomass input relies on surface-derived fluids via tectonic fractures. Despite growing interest in subsurface habitats, little is known about ecosystems beneath arid landscapes, where surface water input is limited by the low annual precipitation. This study compares granitic subsurface environments beneath arid and humid surface ecosystems, highlighting the link between surface climate and subsurface biodiversity. DNA was extracted from granitic subsurface rocks recovered from two endmember sites along a north?south climate gradient in Chile?s Coastal Cordillera. Microbial communities inhabiting down to 55 m deep subsurface rocks were characterized using 16S rRNA amplicon and shotgun metagenomic sequencing. We identified an abundant and potentially active subsurface community below both climates dominated by heterotrophic bacteria, including Pseudarthrobacter, Janthinobacterium, and Pseudomonas. However, rare taxa affiliated with common chemolithoautrophs, e.g. Thiobacillus, Sulfuriferula, and Sulfuricurvum, were only observed in the arid subsurface, indicating increased oligotrophic conditions and reliance on inorganic electron donors in the deeper subsurface of the desert. Functional analysis revealed sulphur, hydrogen, and carbon monoxide as potential inorganic electron donors. These findings expand the current understanding of microbial life in the subsurface of granite rocks showing the influence of surface climate on nutrient conditions in the deeper subsurface, providing new insights into the extent and functional capacity of terrestrial subsurface habitats and their role in global biogeochemical processes. 
pdf:docinfo:custom:PDFVersion: 1.5
pdf:docinfo:subject:  DOI: 10.1093/ismeco/ycaf199, ISME Communications, 5(1), 1, 4 11 2025. Abstract: Subsurface microorganisms face extreme challenges such as anoxic, xeric, and oligotrophic conditions. In igneous systems, nutrient limitation is critical, as biomass input relies on surface-derived fluids via tectonic fractures. Despite growing interest in subsurface habitats, little is known about ecosystems beneath arid landscapes, where surface water input is limited by the low annual precipitation. This study compares granitic subsurface environments beneath arid and humid surface ecosystems, highlighting the link between surface climate and subsurface biodiversity. DNA was extracted from granitic subsurface rocks recovered from two endmember sites along a north?south climate gradient in Chile?s Coastal Cordillera. Microbial communities inhabiting down to 55 m deep subsurface rocks were characterized using 16S rRNA amplicon and shotgun metagenomic sequencing. We identified an abundant and potentially active subsurface community below both climates dominated by heterotrophic bacteria, including Pseudarthrobacter, Janthinobacterium, and Pseudomonas. However, rare taxa affiliated with common chemolithoautrophs, e.g. Thiobacillus, Sulfuriferula, and Sulfuricurvum, were only observed in the arid subsurface, indicating increased oligotrophic conditions and reliance on inorganic electron donors in the deeper subsurface of the desert. Functional analysis revealed sulphur, hydrogen, and carbon monoxide as potential inorganic electron donors. These findings expand the current understanding of microbial life in the subsurface of granite rocks showing the influence of surface climate on nutrient conditions in the deeper subsurface, providing new insights into the extent and functional capacity of terrestrial subsurface habitats and their role in global biogeochemical processes. 
Content-Type: application/pdf
pdf:docinfo:creator: 
X-Parsed-By: org.apache.tika.parser.DefaultParser
dc:language: en
dc:subject: 
meta:creation-date: 2025-11-26T11:25:51Z
created: 2025-11-26T11:25:51Z
access_permission:extract_for_accessibility: true
access_permission:assemble_document: true
xmpTPg:NPages: 17
Creation-Date: 2025-11-26T11:25:51Z
pdf:charsPerPage: 5006
access_permission:extract_content: true
access_permission:can_print: true
meta:keyword: 
producer: Acrobat Distiller 23.0 (Windows); modified using iTextSharp.LGPLv2.Core 3.7.4.0
access_permission:can_modify: true
pdf:docinfo:producer: Acrobat Distiller 23.0 (Windows); modified using iTextSharp.LGPLv2.Core 3.7.4.0
pdf:docinfo:created: 2025-11-26T11:25:51Z